MC38-CAS9 cell line

EN CN
×

Subscribe Us

By subscription, you consent to allow Ubigene Biosciences to store and process the information provided above to deliver the latest news, research spotlight, and promotions. You can unsubscribe from these communications at anytime.
location： Home > Products > Cas9 Stable Cell Lines > MC38-CAS9

EZ-editor™ Gene-editing Series Products

   CRISPR-iScreen™ Library

CRISPR-iScreen™ Library Plasmid

CRISPR-iScreen™ Library Virus

CRISPR-iScreen™ Cell Pool

   gRNA Plasmid Bank

   Cell Lines

KO Cell Lines

Wild-type Cell Lines

Digestive System

Endocrine System

Respiratory System

Blood and Lymphatic System

Urinary System

Reproductive System

Circulatory System

Brain and Nervous System

Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Cas9 Stable Cell Lines

Digestive System

Endocrine System

Respiratory System

Blood and Lymphatic System

Urinary System

Reproductive System

Circulatory System

Brain and Nervous System

Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Luciferase Stable Cell Lines

Digestive System

Endocrine System

Respiratory System

Blood and Lymphatic System

Urinary System

Reproductive System

Circulatory System

Brain and Nervous System

Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

EGFP Stable Cell Lines

Digestive System

Endocrine System

Respiratory System

Blood and Lymphatic System

Urinary System

Reproductive System

Circulatory System

Brain and Nervous System

Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Stem Cell Lines

   Culture Medium

Transfection Culture Medium

Cell Monoclonal Culture Medium

EZ-Stem™ Cell Culture Medium

EZ-cryo™ cell freezing medium

   Kits

Monoclone Genotype Validation Kit

Gene Knockout kit

Mouse Genotyping Kit

Lentiviral Packaging Kit

   Lentivirus

gene KO/PM cell lines
All products
  • All products
  • Red Cotton™ gRNA Plasmid Bank
  • KO Cell Lines
  • Wild-type Cell Lines
  • Cas9 Stable Cell Lines
  • Luciferase Stable Cell Lines
  • EGFP Stable Cell Lines
  • Stem Cell Lines
  • Transfection Culture Medium
  • Cell Monoclonal Culture Medium
  • EZ-Stem™ Cell Culture Medium
  • CRISPR-iScreen™ Library Plasmid
  • CRISPR-iScreen™ Library Virus
  • CRISPR-iScreen™ Cell Pool
  • EZ-cryo™ cell freezing medium
  • Kits
  • Lentivirus

MC38-CAS9 Order

MC38-CAS9 cell line
Catalog#
YC-A002-Cas9-H
Size
1*10^6
Transfection Culture Medium
Transfection Culture medium-A
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Datasheet
MC38-CAS9 cell line
Order

Detailed Product Information

Product category
Cas9 Stable Cell Lines
cell name
MC38-CAS9
Cell Morphology
Epithelial-like, Mixed: adherent and suspensio
Passage ratio
1：2-1：4
Species
Mouse
Expression gene
Cas9
Resistance gene
hygro
Construction method
Lentivirus
Mycoplasma test
negative
Culture method
90% DMEM+10%FBS
Description
Use Cas9 gene expressing lentivirus constructed by Ubigene to infect the MC38 cell line， screen the cells based on the resistance marker and the optimal antibiotic screening concentration explored in advance, resulting in a MC38-CAS9 cell line that can maintain long-term, stable and high expression.
Product Validation Data（RT-QPCR）
Sample Name Target Name Cт Mean ΔCт
MC38-CAS9 Cas9 14.14247417 0.22941589
MC38-CAS9 β-actin 13.91305828
MC38 Cas9 32.94183350 19.78059006
MC38 β-actin 13.16124344
Cutting Efficiency Validation
MC38-CAS9 cell line Product Validation Data（RT-QPCR）
Note: The above figure shows the sequencing peaks of the MC38-CAS9 stable cell pool which is electroporated by Cd47 gene targeting gRNA plasmid, after 48h antibiotic screening. The red arrow indicates the position where the nested peak appears, which shows that the genotype of the target site is significantly changed due to the cutting. Therefore, it indicates that Cas9 nuclease is successfully expressed.
STR Profile
Click to download the STR appraisal report
Loci STR Info (Sample Cell)
Sample Cell Line:MC38		 STR Info (Cell bank)
Cell Line:MC-38[EXPASY kerafast]
Allele1Allele2Allele3Allele4Allele1Allele2Allele3
18-3 15.016.015.016.0
4-2 20.320.3
6-7 15.016.015.016.0
19-2 13.013.0
1-2 19.018.019.020.0
7-1 26.226.2
8-1 16.016.0
1-1 16.017.015.016.017.0
3-2 13.014.015.014.015.0
2-1 16.016.0
15-3 22.323.322.323.3
6-4 18.018.0
13-1 17.017.0
11-2 16.016.0
17-2 15.016.015.016.0
12-1 17.017.0
5-5 16.017.016.017.0
X-1 27.027.0
TH01
D5S818
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Product Advantage

  • Easy to use：Cas9 cell lines in our cell bank can stably express Cas9 protein. Each Cas9 Stable Cell Line is easy to use and enables gene knockout simply by transfecting gRNA, while transfection of gRNA and donor DNA results in gene knock-in or point mutations
    01
  • High-efficient KO：These Cas9 stable cell lines have been used to construct the KO cell lines for various genes, gene KO efficiency 5-10 times improved.
    02
  • High-quality cell：Selected cells, low passages, good cell condition, high activity, applicable for all kinds of gene-editing experiments.
    03
MC38-CAS9 cell line
MC38-CAS9
Catalog#
YC-A002-Cas9-H
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

Related Popular Products

KO Cell Lines

  • Pdcd1 Knockout cell line (MC38)

    Pdcd1 Knockout cell line (MC38)
      inquiry   More details

    Organism: Mouse
    Gene Name: Pdcd1
    Gene ID: 18566
    Size: 1*10^6
    Catalog#: YKO-M241
    YKO-M241(MC38)
  • H2-K1 Knockout cell line (MC38)

    H2-K1 Knockout cell line (MC38)
      inquiry   More details

    Organism: Mouse
    Gene Name: H2-K1
    Gene ID: 14972
    Size: 1*10^6
    Catalog#: YKO-M239
    YKO-M239(MC38)
  • Ncam1 Knockout cell line (MC38)

    Ncam1 Knockout cell line (MC38)
      inquiry   More details

    Organism: Mouse
    Gene Name: Ncam1
    Gene ID: 17967
    Size: 1*10^6
    Catalog#: YKO-M238
    YKO-M238(MC38)

Related Popular Products

KO Cell Lines

  • Pdcd1 Knockout cell line (MC38)

    Pdcd1 Knockout cell line (MC38)

    Organism: Mouse
    Gene Name: Pdcd1
    Gene ID: 18566
    Size: 1*10^6
    Catalog#: YKO-M241
      inquiry   More details
  • H2-K1 Knockout cell line (MC38)

    H2-K1 Knockout cell line (MC38)

    Organism: Mouse
    Gene Name: H2-K1
    Gene ID: 14972
    Size: 1*10^6
    Catalog#: YKO-M239
      inquiry   More details
  • Ncam1 Knockout cell line (MC38)

    Ncam1 Knockout cell line (MC38)

    Organism: Mouse
    Gene Name: Ncam1
    Gene ID: 17967
    Size: 1*10^6
    Catalog#: YKO-M238
      inquiry   More details

WhatsApp: +86 153 6067 3248
TEL: +86 153 6067 3248 (Int'l)
US Toll free: 855 777 3210
EU Toll free: 800 3272 9252
Korea Toll free: 001 800 3272 9252

Gene Editing Services

EZ-editor™ Products

Copyright © 2024 Ubigene. All rights reserved.
Contact us